diff --git a/Marlin/src/sd/Sd2Card.h b/Marlin/src/sd/Sd2Card.h
index 2cec7f3a2..cc895b009 100644
--- a/Marlin/src/sd/Sd2Card.h
+++ b/Marlin/src/sd/Sd2Card.h
@@ -90,23 +90,6 @@ uint8_t const SD_CARD_TYPE_SD1  = 1,                    // Standard capacity V1
 //
 #define SD_CHIP_SELECT_PIN SS_PIN
 
-#if 0
-#if DISABLED(SOFTWARE_SPI)
-  // hardware pin defs
-  #define SD_CHIP_SELECT_PIN SS_PIN   // The default chip select pin for the SD card is SS.
-  // The following three pins must not be redefined for hardware SPI.
-  #define SPI_MOSI_PIN MOSI_PIN       // SPI Master Out Slave In pin
-  #define SPI_MISO_PIN MISO_PIN       // SPI Master In Slave Out pin
-  #define SPI_SCK_PIN SCK_PIN         // SPI Clock pin
-#else  // SOFTWARE_SPI
-  #define SD_CHIP_SELECT_PIN SOFT_SPI_CS_PIN  // SPI chip select pin
-  #define SPI_MOSI_PIN SOFT_SPI_MOSI_PIN      // SPI Master Out Slave In pin
-  #define SPI_MISO_PIN SOFT_SPI_MISO_PIN      // SPI Master In Slave Out pin
-  #define SPI_SCK_PIN SOFT_SPI_SCK_PIN        // SPI Clock pin
-#endif  // SOFTWARE_SPI
-
-#endif
-
 /**
  * \class Sd2Card
  * \brief Raw access to SD and SDHC flash memory cards.
diff --git a/Marlin/src/sd/SdFatConfig.h b/Marlin/src/sd/SdFatConfig.h
index feaafcdc2..8ab6aef1c 100644
--- a/Marlin/src/sd/SdFatConfig.h
+++ b/Marlin/src/sd/SdFatConfig.h
@@ -90,12 +90,6 @@
 // Set USE_SOFTWARE_SPI nonzero to ALWAYS use Software SPI.
 #define USE_SOFTWARE_SPI 0
 
-// Define software SPI pins so Mega can use unmodified 168/328 shields
-#define SOFT_SPI_CS_PIN   10 // Software SPI chip select pin for the SD
-#define SOFT_SPI_MOSI_PIN 11 // Software SPI Master Out Slave In pin
-#define SOFT_SPI_MISO_PIN 12 // Software SPI Master In Slave Out pin
-#define SOFT_SPI_SCK_PIN  13 // Software SPI Clock pin
-
 /**
  * The __cxa_pure_virtual function is an error handler that is invoked when
  * a pure virtual function is called.
diff --git a/Marlin/src/sd/usb_flashdrive/Sd2Card_FlashDrive.h b/Marlin/src/sd/usb_flashdrive/Sd2Card_FlashDrive.h
index 6e7cc82ff..27257b947 100644
--- a/Marlin/src/sd/usb_flashdrive/Sd2Card_FlashDrive.h
+++ b/Marlin/src/sd/usb_flashdrive/Sd2Card_FlashDrive.h
@@ -46,15 +46,8 @@
 #if DISABLED(SOFTWARE_SPI)
   // hardware pin defs
   #define SD_CHIP_SELECT_PIN  SS_PIN            // The default chip select pin for the SD card is SS.
-  // The following three pins must not be redefined for hardware SPI.
-  #define SPI_MOSI_PIN        MOSI_PIN          // SPI Master Out Slave In pin
-  #define SPI_MISO_PIN        MISO_PIN          // SPI Master In Slave Out pin
-  #define SPI_SCK_PIN         SCK_PIN           // SPI Clock pin
 #else  // SOFTWARE_SPI
   #define SD_CHIP_SELECT_PIN  SOFT_SPI_CS_PIN   // SPI chip select pin
-  #define SPI_MOSI_PIN        SOFT_SPI_MOSI_PIN // SPI Master Out Slave In pin
-  #define SPI_MISO_PIN        SOFT_SPI_MISO_PIN // SPI Master In Slave Out pin
-  #define SPI_SCK_PIN         SOFT_SPI_SCK_PIN  // SPI Clock pin
 #endif // SOFTWARE_SPI
 
 class Sd2Card {